Author(s)

Xiao-mei Li, Xue-ping Liu, Yun-qing Ma, Wei-wei Huang

Corresponding Author

Wei-wei Huang

Abstract

Objective: To investigate the effect of porphyrin compound TMPyP4 on autophagy of tumor cells and the underlying molecular mechanism. Methods: the effect of TMPyP4 on autophagy related protein expression and autophagy body formation was detected by immunoblotting and fluorescence. Results: TMPyP4 activate autophagy flow and up-regulate autophagy level in tumor cells; TMPyP4 induced autophagy dependents on AMPK signal pathway; inhibition of autophagy enhances the cytotoxic effect of TMPyP4 on tumor cells. Conclusion: the above results indicate that TMPyP4 induce protective autophagy in tumor cell by activating AMPK signal pathway, suggesting that combination with autophagy inhibitors can improve the antitumor effect of TMPyP4 and its mediated photodynamic therapy.

Keywords

Tmpyp4; Cancer Cells; Autophagy; AMPK