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Molecular Study of Clinical Carbapenem Resistant Providencia Rettgeri

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DOI: 10.38007/Proceedings.0002006


Cha Xu, Hongqin Wang, Bo Feng and Chenxiao Li

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Cha Xu


Abstract: Objective: To investigate the genotyping, drug resistance phenotype and homology of carbapenem resistant Providencia rettgeri. Methods: The strain identification and antibiotic susceptibilities tests of carbapenem-resistant Providencia rettger were caarried out by using VITEK-2 compact system (BioMerieux). The genotypes of genes were screened by PCR. Conjugation experiments were performed to determine the transferability of Plasmids. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). Results: Modified Hodge tests of 9 strains were all positive. The 9 isolates of carbapenem resistant Providencia rettgeri carried blaNDM-1and blaTEM116, one isolate also harbored aac(6′)-Ⅰb, the drug resistance showed multi-drug resistance. One strain of the NDM-1 gene were successfully transferred to recipient bacteria(E. coli J53AziR) by conjugation. PFGE analysis showed higly homology of the 9 isolates. Conclusion: The NDM-1 producing Providencia rettgeri isolates in this study appeared to be clonal, hospitals should strengthen the monitoring of carbapenem-resistant bacteria.With the emergence of a large number of carbapenem-resistant enterobacteriaceae bacteria, bacterial infections caused by them maintains an upward trend all over the world [1]. The result on the bacterial drug resistance showed in 2016 that Klebsiella pneumoniae was the main carbapenem-resistant bacteria, with drug resistance rate of >15%.At present, few studies have been reported on Providencia Rettgeri that is drug resistant to carbapeneme. In this study, clinical resistance and molecular epidemiology of the clinically isolated carbapene-resistant Providencia Rettgeri were analyzed.


Keywords: Providencia Rettgeri; Carbapenem Resistance; Clinical distribution; Homology