The Screening Method of Chinese Herbal Medicine Activity Based on Affinity Chromatography
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DOI: 10.38007/Proceedings.0000233
Author(s)
Yani Hou, Congshan Shang and Tingting Meng
Corresponding Author
Yani Hou
Abstract
Drug inhibitors are commonly used in drugs to treat human diseases. China is rich in resources of traditional Chinese medicine. Screening low toxicity and high efficiency drug inhibitors from traditional Chinese medicine is of great significance for discovering innovative lead compounds and creating new drugs with independent intellectual property rights. The aim of this paper is to use affinity ultrafiltration and liquid-mass technology to screen the target enzyme inhibitors in Chinese herbal medicine, providing a new idea and method for the rapid screening and identification of the target enzyme inhibitors in the complex system of Chinese traditional medicine. The acetylcholinesterase affinity ultrafiltration system was established by optimizing the culture conditions and chromatographic conditions of affinity ultrafiltration. The established system was used to screen and identify 5 compounds with significant inhibitory effects from 80% ethanol extracts of rhizoma coptidis. The active compounds were prepared by semi-preparative liquid phase and identified by mass spectrometry and nuclear magnetic resonance spectroscopy. The conclusion of this study was that the free radical scavenging ability of traditional Chinese medicine extract with good tyrosinase inhibitory activity was detected, and its IC50 values were 45.55, 298.87, 1096.6 and 992.27 mgL-1, respectively.
Keywords
Chinese Herbal medicine; Affinity Chromatography; Acetylcholinesterase; Drug Activity; Affinity Ultrafiltration with Liquid mass